Amplification of DNA from whole blood.

Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples

Potent PCR inhibitors in blood and soil samples can cause false negative results from PCR-based clinical and forensic tests. We show that the effect of these inhibitors is primarily upon Taq DNA polymerase, since mutational alteration of the polymerase can overcome the inhibition to the extent that no DNA purification is now required. An N-terminal deletion (Klentaq1) is some 10-100-fold inhibi...

Detection of Trypanosoma Evansi in Whole Blood of Domestic Animals by Dna Amplification Method

Trypanosoma evansi, a blood protozoan parasite causing ‘surra’ in domestic animals is widely prevalent in different parts and is of significant economic importance in livestock production. Parasitaemia is often intermittent and is not always possible to demonstrate the parasites in blood and hence diagnosis of trypanosomosis is often difficult. The present investigation has been carried out wit...

Polymerase chain reaction amplification of Trypanosoma cruzi kinetoplast minicircle DNA isolated from whole blood lysates: diagnosis of chronic Chagas' disease.

A 6 M guanidine-HCl/0.2 M EDTA solution was used to lyse and store whole blood specimens. DNA stored in guanidine-EDTA-blood (GEB) lysate was found to be undegraded after incubation at 37 degrees C for 1 month, suggesting that this represents an appropriate reagent for transport of blood samples from the field to a laboratory for analysis. Trypanosoma cruzi kinetoplast DNA in GEB lysate can be ...

Isolation of genomic DNA from human whole blood.

Blood is the main source of DNA for genotype-related studies in humans. A rapid, efficient, and cost-effective method for the isolation of genomic DNA from whole blood is needed for screening a large number of samples. There are many published protocols (1,3–7). These procedures use toxic organic chemicals (SDS-proteinase Kphenol isolation method or its modifications) (1,3) and/or are time cons...

Completely automated extraction of DNA from whole blood.